Manual of diagnostic tests for aquatic animals

Valid laboratory results are essential for Aquatic Animal Health Services to implement a range of programmes for diagnosis, surveillance and trade. This Manual of Diagnostic Tests for Aquatic Animals (Aquatic Manual) supports Aquatic Animal Health Services to provide effective laboratory testing for pathogenic agents that may adversely affect aquatic animals. (...) The World Organisation for Animal Health … Continue reading Manual of diagnostic tests for aquatic animals

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Haemocytes from Crassostrea gigas and OsHV-1: A promising in vitro system to study host/virus interactions

Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate … Continue reading Haemocytes from Crassostrea gigas and OsHV-1: A promising in vitro system to study host/virus interactions

Oyster RNA-seq data support the development of Malacoherpesviridae genomics

The family of double-stranded DNA (dsDNA) Malacoherpesviridae includes viruses able to infect marine mollusks and detrimental for worldwide aquaculture production. Due to fast-occurring mortality and a lack of permissive cell lines, the available data on the few known Malacoherpesviridae provide only partial support for the study of molecular virus features, life cycle, and evolutionary history. … Continue reading Oyster RNA-seq data support the development of Malacoherpesviridae genomics

A novel monoclonal Perkinsus chesapeaki in vitro isolate from an Australian cockle, Anadara trapezia

A monoclonal Perkinsus chesapeaki isolate was established from 1 of 10 infected Australian Anadara trapezia cockles. Morphological features were similar to those of described P. chesapeaki isolates, and also included a unique vermiform schizont cell-type. Perkinsus olseni-specific PCR primers amplified DNAs from all 10 cockles. Perkinsus chesapeaki-specific primers also amplified DNAs from 4/10 cockles, including … Continue reading A novel monoclonal Perkinsus chesapeaki in vitro isolate from an Australian cockle, Anadara trapezia

The interaction between algal viruses and the mussel Mytilus galloprovincialis Lamarck, 1819 (Bivalvia: Mytilidae) in experiments

The utilization (possible assimilation and concentration in organs) of 99% of algal viruses from the water by the Black Sea mussel Mytilus galloprovincialis was confirmed experimentally. This index depends on the duration of the experiment, the complexity of the morphology of the virus, the age (size) of the mussels, and the presence of a nonindicatory … Continue reading The interaction between algal viruses and the mussel Mytilus galloprovincialis Lamarck, 1819 (Bivalvia: Mytilidae) in experiments

Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat

High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study … Continue reading Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat

Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections

Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to … Continue reading Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections