Mass selection for survival and resistance to OsHV-1 infection in Crassostrea gigas spat in field conditions: response to selection after four generations

The ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster Crassostrea gigas, and numerous mortality outbreaks have been observed worldwide. We report the first results of our experimental breeding program using mass selection focused on survival and resistance to OsHV-1 after four generations of selection for two lines. These lines … Continue reading Mass selection for survival and resistance to OsHV-1 infection in Crassostrea gigas spat in field conditions: response to selection after four generations

Distribution of Ostreid herpesvirus-1 (OsHV-1) microvariant in seawater in a recirculating aquaculture system

Ostreid herpesvirus-1 (OsHV-1) microvariant presents a significant threat to the commercial production of Crassostrea gigas worldwide. Investigating the transmission of the virus in the estuarine environment where oysters are grown has proven to be difficult due to the complexity of the ecosystem. The aims of this trial were to (1) investigate the distribution of OsHV-1 … Continue reading Distribution of Ostreid herpesvirus-1 (OsHV-1) microvariant in seawater in a recirculating aquaculture system

The use of size and growing height to improve Crassostrea gigas farming and breeding techniques against OsHV-1

Mortality associated with ostreid herpesvirus 1 (OsHV-1) has significantly impacted Crassostrea gigas farmers all around the world. Although selective breeding programs to improve OsHV-1 resistance can significantly reduce the mortality rate below 20%, husbandry practices have also been one way to limit or decrease mortality. The main objectives of this study were to describe the … Continue reading The use of size and growing height to improve Crassostrea gigas farming and breeding techniques against OsHV-1

Detection of Ostreid herpesvirus-1 microvariants in healthy Crassostrea gigas following disease events and their possible role as reservoirs of infection

Ostreid herpesvirus-1 microvariants (OsHV-1) cause severe mortalities in farmed Crassostrea gigas in Europe, New Zealand and Australia. Outbreaks are seasonal, recurring in the warmer months of the year in endemic estuaries. The reference genotype and microvariant genotypes of OsHV-1 have been previously detected in the tissues of apparently healthy adult oysters naturally exposed to OsHV-1 … Continue reading Detection of Ostreid herpesvirus-1 microvariants in healthy Crassostrea gigas following disease events and their possible role as reservoirs of infection

Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis

Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this … Continue reading Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis

Transmission of Ostreid herpesvirus-1 microvariant in seawater: Detection of viral DNA in seawater, filter retentates, filter membranes and sentinel Crassostrea gigas spat in upwellers

To understand the transmission of Ostreid herpesvirus-1 microvariant(s) (OsHV-1) in seawater, it is necessary to understand the temporal pattern of occurrence of the virus in seawater in relation to demonstrated infection events in oysters. The aims of the current study were: (1) to investigate the temporal sequence of detection of OsHV-1 in seawater and Crassostrea … Continue reading Transmission of Ostreid herpesvirus-1 microvariant in seawater: Detection of viral DNA in seawater, filter retentates, filter membranes and sentinel Crassostrea gigas spat in upwellers

Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat

High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study … Continue reading Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat