Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix

Selecting fast-growing strains is the most common objective of genetic breeding programs. In this study, we sought to mine the growth-associated single nucleotide polymorphisms (SNPs) in the commercially cultured clam Meretrix meretrix by candidate gene association analysis. Fifty potentially growth-associated contigs based on their functional annotation were chosen as candidates from our EST database, and … Continue reading Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix

Ancient DNA analysis identifies marine mollusc shells as new metagenomic archives of the past

Marine mollusc shells enclose a wealth of information on coastal organisms and their environment. Their life history traits as well as (palaeo-) environmental conditions, including temperature, food availability, salinity and pollution, can be traced through the analysis of their shell (micro-) structure and biogeochemical composition. Adding to this list, the DNA entrapped in shell carbonate … Continue reading Ancient DNA analysis identifies marine mollusc shells as new metagenomic archives of the past

Species composition and genetic diversity of farmed mussels in British Columbia, Canada

The common blue mussel (Mytilus edulis) was introduced to British Columbia, Canada, in the 1980s as an aquaculture alternative to native mussel species. Since then, the mussel industry in Pacific Canada has expanded and includes operations utilizing traditional methods of broodstock selection based on visual qualitative and quantitative traits. The impacts of hatchery propagation on … Continue reading Species composition and genetic diversity of farmed mussels in British Columbia, Canada

Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture

The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17% and 98% mortality in blue mussel larvae in a … Continue reading Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture

Construction and evaluation of a high-density SNP array for the Pacific oyster (Crassostrea gigas)

Single nucleotide polymorphisms (SNPs) are widely used in genetics and genomics research. The Pacific oyster (Crassostrea gigas) is an economically and ecologically important marine bivalve, and it possesses one of the highest levels of genomic DNA variation among animal species. Pacific oyster SNPs have been extensively investigated; however, the mechanisms by which these SNPs may … Continue reading Construction and evaluation of a high-density SNP array for the Pacific oyster (Crassostrea gigas)

Validation of reference genes for RT-qPCR in marine bivalve ecotoxicology: Systematic review and case study using copper treated primary Ruditapes philippinarum hemocytes

The appropriate selection of reference genes for the normalization of non-biological variance in reverse transcription real-time quantitative PCR (RT-qPCR) is essential for the accurate interpretation of the collected data. The use of multiple validated reference genes has been shown to substantially increase the robustness of the normalization. It is therefore considered good practice to validate … Continue reading Validation of reference genes for RT-qPCR in marine bivalve ecotoxicology: Systematic review and case study using copper treated primary Ruditapes philippinarum hemocytes