Highlights • Previous studies of housekeeping genes selection in mollusc species were reviewed. • Ten candidate housekeeping genes were assessed in ark shell under given conditions. • RL7 and EF-1a were the most stable genes across tissues in ark shells. • RL15 appeared more stable than others under a variety of given conditions. • 18s … Continue reading Validation of housekeeping genes for quantitative mRNA expression analysis in OsHV-1 infected ark clam, Scapharca broughtonii
Marine herpesviruses are responsible for epizootics in economically, ecologically and culturally significant taxa. The recent emergence of microvariants of Ostreid herpesvirus 1 (OsHV-1) in Pacific oysters Crassostrea gigas has resulted in socioeconomic losses in Europe, New Zealand and Australia however, there is no information on their origin or mode of transmission. These factors need to … Continue reading Counting the dead to determine the source and transmission of the marine herpesvirus OsHV-1 in Crassostrea gigas
A mortality episode (>90%) of triploid and diploid Pacific oysters Crassostrea gigas cultured in Baja California Sur occurred during summer 2012, coinciding with a thermal anomaly, an algal bloom, and low oxygen values. To help explain the cause of the mortalities, histological analyses and molecular tests for specific pathogens (ostreid herpesvirus 1 [OsHV‐1] and Perkinsus … Continue reading Histological alterations in Pacific oysters Crassostrea gigas that survived a summer mortality event in Baja California, Mexico
An understanding of husbandry strategies and any associated risk factors is important for designing management control measures that can reduce mortality in Pacific oysters, Crassostrea gigas, caused by ostreid herpesvirus 1 (OsHV‐1). The type of culture facility can be considered in relation to the potential pathways that could lead to the entry of a pathogen … Continue reading A literature review as an aid to identify strategies for mitigating ostreid herpesvirus 1 in Crassostrea gigas hatchery and nursery systems
Vibrio coralliilyticus is a bacterial pathogen which can affect a range of marine organisms, such as corals, fish and shellfish, with sometimes devastating consequences. However, little is known about the mechanisms involved in the host-pathogen interaction, especially within molluscan models. We applied gas chromatography-mass spectrometry (GC-MS)-based metabolomics to characterize the physiological responses in haemolymph of … Continue reading Metabolomics study of immune responses of New Zealand Greenshell™ mussels (Perna canaliculus) infected with pathogenic Vibrio sp.
The aquaculture sector is significantly behind plant and farm animal production in applying selective breeding, in spite of the fact that it has been suggested that the world aquaculture production could be doubled in 13 years if breeding programmes were supplying stocks for the farmed species. It is estimated that as late as in 2010, only … Continue reading Selection response in fish and shellfish: a review
Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European farming areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus clade. Ruling out the complexity … Continue reading Inefficient immune response is associated with microbial permissiveness in juvenile oysters affected by mass mortalities on field